Separation and Fractionation

Culsma separates material with sep(...) and creates ordered fractions with frac(...).

Both forms take a sample and a program.

sep(...)

sep(...) splits one container into a fixed two-slot group.

let g = sep(sample = lysate, program = centrifuge_program(drive = 12000g));

The result is a sep_container_group. Access its slots with group[0] and group[1].

output << [g[0]:40uL];

Separation Programs

sep(...) accepts mechanism-specific programs.

Program	Required fields	Optional fields	Meaning
centrifuge_program(...)	drive	keep_source	Centrifuge separation.
magnetic_program(...)	none	duration, device	Magnetic separation.
disrupt_program(...)	none	duration	Disruption or lysis-oriented separation.
field_program(...)	field	duration	Field-driven separation.
filtration_program(...)	membrane, drive	none	Filtration separation.
phase_partition_program(...)	solvent	none	Phase partition.
precipitation_program(...)	reagent	duration	Precipitation.

Slot Meaning

The two slots have stable meanings determined by the program.

Program	group[0]	group[1]
centrifuge_program	supernatant	pellet
magnetic_program	bound	flowthrough
disrupt_program	lysate	debris_or_residue
field_program	target_band_fraction	non_target_fraction
filtration_program	filtrate	retentate
phase_partition_program	target_phase	other_phase
precipitation_program	precipitate	supernatant

keep_source

keep_source is only for centrifuge_program(...).

let g = sep(
    sample = lysate,
    program = centrifuge_program(drive = 12000g, keep_source = pellet)
);

keep_source = supernatant reuses the input container for group[0]. keep_source = pellet reuses the input container for group[1].

If keep_source is omitted, both slots are new containers.

frac(...)

frac(...) creates an ordered fraction group.

let fg = frac(
    sample = tube,
    program = density_gradient_program(axis = "density", order = "top_to_bottom", bins = 8)
);

The result is a fraction_group. Access slots with fg[0], fg[1], and so on.

Fractionation Programs

Program	Required fields	Meaning
density_gradient_program(...)	axis, order, bins	Density or layer-based fractionation.
chromatography_program(...)	axis, order, bins	Retention-order fractionation.

Index Rules

Group indexes are zero-based.

• sep_container_group supports only group[0] and group[1].
• fraction_group[i] must be within 0 <= i < bins when bins is known.
• Slices, multidimensional indexes, and dynamic index semantics are not part of this authoring surface.